Multimarker Reverse Transcriptase-Polymerase Chain Reaction Assay in Lymphatic Drainage and Sentinel Node Tumor Burden
نویسندگان
چکیده
منابع مشابه
Reverse transcriptase-polymerase chain reaction (rt-PCR).
Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene expression studies. Since its introduction (...
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The real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) has become the method of choice for the quantification of specific mRNAs. This method is fast, extremely sensitive, and accurate, requires only very small amounts of input RNA, and is relatively simple to perform. These characteristics have made it the method of choice for minimal residual disease monitoring suc...
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A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecti...
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Many genes that play a major physiologic role present low levels of expression, whose characterization requires accurate quantitation methods of adequate sensitivity. The use of the reverse-transcriptase polymerase chain reaction (RT-PCR) coupled to capillary electrophoresis (CE) for the analysis of steady-state messenger RNA levels has become increasingly widespread in recent years [1–5]. The ...
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ژورنال
عنوان ژورنال: Annals of Surgical Oncology
سال: 2010
ISSN: 1068-9265,1534-4681
DOI: 10.1245/s10434-010-1142-9